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Further, SOD, a permeating cryoprotectant, acts as an antioxidant and causes membrane lipid and protein rearrangement, which results in increased membrane fluidity, greater dehydration at lower temperatures, and therefore increased ability of spermatozoa to survive during this preservation [ 50 ]. We analysed sodium Na , copper Cu and selenium Se levels in human semen and glutathione peroxidase activity GPx in seminal plasma and examined their relationships with sperm quality. On the other hand, a significant improvement in sperm parameter recovery was seen in the aliquot with both SOD and catalase supplementation; perhaps because of their combined and simultaneous action on superoxide anion and hydrogen peroxide. Drobnis EZ,Crowe LM,Berger T,Anchordoguy TJ,Overstreet JW andCrowe JH Cold shock damage is due to lipid phase transitions in cell membranes: J Clin Lab Anal. Postepy Hig Med Dosw Author information 1 Instituto de Biologia y Medicina Experimental, CONICET, Buenos Aires, Argentina.
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Hydrogen peroxide produced by superoxide dismutase SOD-2 activates sperm in Caenorhabditis elegans

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Free Radic Biol Med. Abstract The study was conducted to determine the influence of N-acetyl- l -cysteine NAC and superoxide dismutase SOD on chicken sperm motility, plasma membrane and acrosome integrity, mitochondrial activity, lipid peroxidation LPO and apoptotic changes after freezing—thawing process. Moreover, the H 2 O 2 scavenger ebselen completely inhibited pseudopod extension in wild-type sperm treated with Pronase, and H 2 O 2 could directly induce pseudopod extension in wild-type sperm. During the study, all the experimental protocols met the Institutional Animal Care and Use Committee regulations.
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